# WetLab Protocols

- [无菌操作原则](/liulab-docs/wetlab-protocols/wu-jun-cao-zuo-yuan-ze.md): By 实验老司机
- [细胞培养注意事项](/liulab-docs/wetlab-protocols/xi-bao-pei-yang-zhu-yi-shi-xiang.md)
- [常用细胞操作](/liulab-docs/wetlab-protocols/chang-yong-xi-bao-cao-zuo.md): By KaiyiFu
- [质粒转化、扩增、提取](/liulab-docs/wetlab-protocols/zhi-li-zhuan-hua-kuo-zeng-ti-qu.md): By GYY, Kaiyi
- [PCR+琼脂糖电泳](/liulab-docs/wetlab-protocols/pcr+-qiong-zhi-tang-dian-yong.md): By Kaiyi
- [分子克隆-无缝克隆](/liulab-docs/wetlab-protocols/fen-zi-ke-long-wu-feng-ke-long.md): By Yiyuan Gao, Yuedi Wang & Kaiyi Fu
- [分子克隆-同源重组（质粒点突变、截短）](/liulab-docs/wetlab-protocols/fen-zi-ke-long-tong-yuan-zhong-zu-zhi-li-dian-tu-bian-jie-duan.md): By Frederick & Mariana
- [重组蛋白原核表达](/liulab-docs/wetlab-protocols/zhong-zu-dan-bai-yuan-he-biao-da.md): By Kaiyi Fu
- [慢病毒包装与转导](/liulab-docs/wetlab-protocols/man-bing-du-bao-zhuang-yu-zhuan-dao.md): By Kaiyi & Yiyuan
- [脂质体转染](/liulab-docs/wetlab-protocols/zhi-zhi-ti-zhuan-ran.md): By Kaiyi Fu
- [CRISPR-KO（慢病毒）](/liulab-docs/wetlab-protocols/crisprko-man-bing-du.md): By Kaiyi
- [CRISPR-KI & CRISPR-a/i](/liulab-docs/wetlab-protocols/crispr-ki-and-crispr-a-i.md): By Kaiyi Fu
- [CRISPR-KO/KI（RNP电转染）](/liulab-docs/wetlab-protocols/crisprkokirnp-dian-zhuan-ran.md): By Kaiyi
- [RT-qPCR](/liulab-docs/wetlab-protocols/rt-qpcr.md): By Kaiyi
- [Western Blot](/liulab-docs/wetlab-protocols/western-blot.md): By Jiayue & Kaiyi
- [斑点印迹（Dot blot）](/liulab-docs/wetlab-protocols/ban-dian-yin-ji-dot-blot.md): By Kaiyi
- [免疫共沉淀 (Co-IP)](/liulab-docs/wetlab-protocols/mian-yi-gong-chen-dian-coip.md): By Frederick & Marriana
- [蛋白临近标记（TurboID）](/liulab-docs/wetlab-protocols/dan-bai-lin-jin-biao-ji-turboid.md): By Kaiyi
- [酶联免疫吸附试验（ELISA）](/liulab-docs/wetlab-protocols/mei-lian-mian-yi-xi-fu-shi-yan-elisa.md): By KY
- [免疫荧光（IF）+共聚焦](/liulab-docs/wetlab-protocols/mian-yi-ying-guang-if+-gong-ju-jiao.md): By Kaiyi, G.T., YBC
- [人原代T细胞分离培养](/liulab-docs/wetlab-protocols/ren-yuan-daitxi-bao-fen-li-pei-yang.md)
- [T细胞细胞毒性实验](/liulab-docs/wetlab-protocols/t-xi-bao-xi-bao-du-xing-shi-yan.md): By Kaiyi
- [CCK-8细胞增殖毒性](/liulab-docs/wetlab-protocols/cck8-xi-bao-zeng-zhi-du-xing.md)
- [Transwell+划痕实验](/liulab-docs/wetlab-protocols/transwell+-hua-hen-shi-yan.md): By Kaiyi
- [肿瘤球+3D侵袭+克隆形成](/liulab-docs/wetlab-protocols/zhong-liu-qiu-+3d-qin-xi-+-ke-long-xing-cheng.md): By Kaiyi
- [细胞黏附实验](/liulab-docs/wetlab-protocols/xi-bao-nian-fu-shi-yan.md): By K.Y.
- [Incucyte细胞功能检测](/liulab-docs/wetlab-protocols/incucyte-xi-bao-gong-neng-jian-ce.md): By Kaiyi
- [Seahorse代谢检测](/liulab-docs/wetlab-protocols/seahorse-dai-xie-jian-ce.md)
- [代谢流（Metabolic flux）](/liulab-docs/wetlab-protocols/dai-xie-liu-metabolic-flux.md): By Kaiyi
- [微管相关实验](/liulab-docs/wetlab-protocols/wei-guan-xiang-guan-shi-yan.md): By Kaiyi
- [原力子显微镜（AFM）](/liulab-docs/wetlab-protocols/yuan-li-zi-xian-wei-jing-afm.md)
- [肠道/肠癌类器官](/liulab-docs/wetlab-protocols/chang-dao-chang-ai-lei-qi-guan.md): By Kaiyi
- [小鼠肠癌造模](/liulab-docs/wetlab-protocols/xiao-shu-chang-ai-zao-mo.md): By Kaiyi
- [小鼠肿瘤单细胞悬液制备](/liulab-docs/wetlab-protocols/xiao-shu-zhong-liu-dan-xi-bao-xuan-ye-zhi-bei.md): By J.Y. & K.Y
- [流式细胞术+流式分选](/liulab-docs/wetlab-protocols/liu-shi-xi-bao-shu-+-liu-shi-fen-xuan.md)
- [细胞周期、凋亡检测](/liulab-docs/wetlab-protocols/xi-bao-zhou-qi-diao-wang-jian-ce.md): By Kaiyi