By Kaiyi
96孔板铺板2000-3000个细胞,10倍镜,每隔15分钟拍照,持续24h
导出AVI格式视频:Export > As displayed > Next, select the wells to export > Next, select timepoints > Next, select folder, choose type of file: AVI no compression > Export
下载celltraxx插件:https://github.com/borge-holme/celltraxx_download/tree/mainarrow-up-right
按照User Manual进行文件夹设置。C盘下需要有celltraxx_system和celltraxx_data两个文件夹,后者用来存放AVI视频文件。
按照User Manual指引,在ImageJ中安装celltraxx插件
点击ImageJ-Plugins-Macros-celltraxx imagej macro
设置各项参数,包括是否是Wound healing mode,Gaussian filter radius,Smallest cell diameter, Largest cell diameter,Cutting cell diameter等。可以拖动紫色框限定分析的范围(划痕模式可以只选前沿)。
查看自动分割的结果,进行微调以及update。
参考文献:
Automated tracking of cell migration in phase contrast images with CellTraxx
Integrated single-cell and spatial transcriptomics uncover distinct cellular subtypes involved in neural invasion in pancreatic cancer
Last updated 5 months ago